Pine Creek is an 86.5 mile long and the largest tributary of the West Branch Susquehanna River in Potter, Tioga, Lycoming, and Clinton counties in Pennsylvania in the United States.  Within Tioga County, 23.25 river miles of Pine Creek are designated as a Pennsylvania Scenic River.  Its source is in Potter County, flowing southeast 16 miles to Galeton, where it receives its first major tributary, the West Branch Pine Creek. It then flows east 12 mi (19.3 km) to Tioga County and the village of Ansonia, receiving second major tributary, Marsh Creek.  At the village of Blackwell, Pine Creek receives its third major tributary, Babb Creek.  It continues southwest 14 mi (22.5 km) and enters Lycoming County, where it turns southeast for 28 mi (45.0 km) to its mouth. At Waterville it receives its fourth major tributary, Little Pine Creek.

The elevation at the source of Pine Creek is 2420 ft, while the mouth is at an elevation of 520 ft (158 m). The difference in elevation (1900 ft or 580 m) divided by the length of the creek of (86.5 mi or 139 km) gives the average drop in elevation per unit length of creek or relief ratio of 22.0 ft/mi (4.2 m/km). The meander ratio is 1.08, so the creek is fairly straight in its bed.

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